| Gene | Drg2 |
| Chromosome | 11 |
| Deletion Start | 60,468,233 |
| Deletion End | 60,454,683 |
| Deletion Size | 13,551 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,455,221 | 60,455,241 | ACCCGCTCTGTTGTGGTTCAC |
| TUP | 60,455,243 | 60,455,266 | TGTGCAAAGCCCTTGCCAGAGTGA |
| TUR | 60,455,300 | 60,455,320 | TGATGCTTCCTTGGTGTTGTG |
| TDF | 60,467,663 | 60,467,684 | CCCTGACTTTCCTGTCCCATAG |
| TDP | 60,467,688 | 60,467,710 | CACCGCATCCATCGGTCACTGGC |
| TDR | 60,467,739 | 60,467,758 | CAGCTCCTGCCCAGACTTAC |
PCR Primers
| Primer | Sequence |
| SU | GGAGCCTTAAGACTTTCTGCC |
| SD | TTTAGGACACTTTGGCTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18159A-F12 | VGB6 | C57BL/6NTac | |
| 18159A-H12 | VGB6 | C57BL/6NTac | |
| 18159A-A3 | VGB6 | C57BL/6NTac | |
