| Gene | Klf10 |
| Chromosome | 15 |
| Deletion Start | 38,300,591 |
| Deletion End | 38,295,858 |
| Deletion Size | 4,734 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,300,573 | 38,300,591 | CTCAACTTCGGCGCTTCTC |
| TUP | 38,300,546 | 38,300,572 | TCCAGCAAGCTTCGGTAAGTTACCTGA |
| TUR | 38,300,450 | 38,300,467 | TGCAGCCTGGGTTCTCAG |
| TDF | 38,296,045 | 38,296,064 | GTTTGCCTGTCCCATGTGTG |
| TDP | 38,296,020 | 38,296,043 | CCGTCGGTTTATGAGGAGCGACCA |
| TDR | 38,295,975 | 38,295,994 | GGCAGCTTCTTGGCTGATAG |
PCR Primers
| Primer | Sequence |
| SU | GGACTGAAGGCTAGGGTTGG |
| SD | CACACTGCAAAGCTGGTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 142 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18162A-A10 | VGB6 | C57BL/6NTac | |
| 18162A-B2 | VGB6 | C57BL/6NTac | |
| 18162A-G3 | VGB6 | C57BL/6NTac | |
| 18162A-D8 | VGB6 | C57BL/6NTac | |
