| Gene | Sra1 |
| Chromosome | 18 |
| Deletion Start | 36,670,269 |
| Deletion End | 36,667,407 |
| Deletion Size | 2,863 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,670,090 | 36,670,109 | AACGACCCGCCACAATTCTC |
| TUP | 36,670,065 | 36,670,088 | TACGGGCTCCAGACTCAGACTGGT |
| TUR | 36,669,972 | 36,669,992 | GGTACAGGATTCTGCCCACAG |
| TDF | 36,667,621 | 36,667,641 | GGGATGCAGCAGATGACATTC |
| TDP | 36,667,593 | 36,667,619 | CCGATCACTCATGGTTGACCATGTGAC |
| TDR | 36,667,569 | 36,667,588 | CCCACCATCCACTGACTGAC |
PCR Primers
| Primer | Sequence |
| SU | TACTGAAGATGAGCGCTTGC |
| SD | GGAGATACAGCAAAGGGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 138 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18167A-B9 | VGB6 | C57BL/6NTac | |
| 18167A-C3 | VGB6 | C57BL/6NTac | |
| 18167A-C8 | VGB6 | C57BL/6NTac | |
| 18167A-F9 | VGB6 | C57BL/6NTac | |
