| Gene | Dcp1b |
| Chromosome | 6 |
| Deletion Start | 119,200,947 |
| Deletion End | 119,175,296 |
| Deletion Size | 25,652 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,175,707 | 119,175,725 | GCAGCCGGAGTGCGAAATG |
| TUP | 119,175,726 | 119,175,747 | CAGGGCAGGCACCCACCTAAGA |
| TUR | 119,175,796 | 119,175,815 | TGGCCTCCTACATCCAAGTC |
| TDF | 119,200,408 | 119,200,426 | GCCCACCTTGCTTGACCTC |
| TDP | 119,200,429 | 119,200,458 | AGCATTCTGGAAGGGAAGGAGTCTGTACCA |
| TDR | 119,200,471 | 119,200,495 | GGAAGCCTTACAGAACATTCAACAG |
PCR Primers
| Primer | Sequence |
| SU | GCACTCAGGAAATCCCTCAC |
| SD | GGCTCATGCTTTGAGTTATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18169B-H5 | VGB6 | C57BL/6NTac | |
