| Gene | Dmrt1 |
| Chromosome | 19 |
| Deletion Start | 25,509,858 |
| Deletion End | 25,505,857 |
| Deletion Size | 4,002 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,506,390 | 25,506,410 | GGGTTGTTCCGAGCAATGAAG |
| TUP | 25,506,415 | 25,506,436 | ATTCGGGCTGCACACGTGCACC |
| TUR | 25,506,490 | 25,506,511 | GCTGGAAGACGAGGTCAGAATG |
| TDF | 25,509,640 | 25,509,658 | TGTGCTCTGCTGGAGTGTG |
| TDP | 25,509,660 | 25,509,684 | CTTGCTGTCTGTGCTTCCAGGTGGC |
| TDR | 25,509,688 | 25,509,705 | TGGGCCTGCTGTCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | GAGAGAGTTCCTCGTGCTGG |
| SD | TGGACAGAGATGGTACATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18171A-A4 | VGB6 | C57BL/6NTac | |
| 18171A-A6 | VGB6 | C57BL/6NTac | |
| 18171A-A8 | VGB6 | C57BL/6NTac | |
| 18171A-D11 | VGB6 | C57BL/6NTac | |
| 18171A-D1 | VGB6 | C57BL/6NTac | |
| 18171A-D4 | VGB6 | C57BL/6NTac | |
