| Gene | Med24 |
| Chromosome | 11 |
| Deletion Start | 98,728,911 |
| Deletion End | 98,704,850 |
| Deletion Size | 24,062 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,728,177 | 98,728,200 | TGTAGAGTTCTTCCCACCTTTCTG |
| TUP | 98,728,138 | 98,728,165 | TCATATTAGCACGCCAAACACCTTCACC |
| TUR | 98,728,115 | 98,728,135 | GGATCTGGCAAAGAGGCTCAG |
| TDF | 98,705,032 | 98,705,051 | CCCAGAAGCTACCTGTGTCC |
| TDP | 98,705,003 | 98,705,030 | TCTGTCTGTGGCAGGTATCAGAACTGGT |
| TDR | 98,704,944 | 98,704,963 | GCAGGCTGAGGTCTGTGATG |
PCR Primers
| Primer | Sequence |
| SU | CGAGGAAGGTAGAGGCAATG |
| SD | CCCTACACAGGATTATGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18173A-F8 | VGB6 | C57BL/6NTac | |
| 18173A-D7 | VGB6 | C57BL/6NTac | |
| 18173A-C3 | VGB6 | C57BL/6NTac | |
