| Gene | Ucp2 |
| Chromosome | 7 |
| Deletion Start | 100,499,424 |
| Deletion End | 100,496,826 |
| Deletion Size | 2,599 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,497,250 | 100,497,268 | CGTCCGCATTGGCCTCTAC |
| TUP | 100,497,272 | 100,497,298 | TCTGTCAAACAGTTCTACACCAAGGGC |
| TUR | 100,497,308 | 100,497,327 | TCTGTCCTTGCCGCATACTC |
| TDF | 100,499,053 | 100,499,073 | AGTGGTGGAGGGCTTGCTTAG |
| TDP | 100,499,074 | 100,499,099 | CATGCTTGGAGACCCTGGGTTCTGCT |
| TDR | 100,499,135 | 100,499,153 | GCCACATGCCCAAGAATCC |
PCR Primers
| Primer | Sequence |
| SU | GTCTCTGCCAGGAGATCAGG |
| SD | TGTTACAGGCAGAATGTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18175A-B4 | VGB6 | C57BL/6NTac | |
| 18175A-B8 | VGB6 | C57BL/6NTac | |
| 18175A-E6 | VGB6 | C57BL/6NTac | |
| 18175A-H4 | VGB6 | C57BL/6NTac | |
| 18175A-A3 | VGB6 | C57BL/6NTac | |
