| Gene | Afmid |
| Chromosome | 11 |
| Deletion Start | 117,838,601 |
| Deletion End | 117,825,953 |
| Deletion Size | 12,649 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,826,294 | 117,826,311 | GCACCTTCAGGGACAGAC |
| TUP | 117,826,315 | 117,826,340 | ATTGAGCGGCTTCAGAATTCCCATGC |
| TUR | 117,826,349 | 117,826,367 | TGCTCGGTGTGAGCCATAG |
| TDF | 117,838,175 | 117,838,196 | GGATCAGACTCAGGCAGTCAAC |
| TDP | 117,838,198 | 117,838,224 | TGGTGGCAAGCACCTTTATCCACTGAG |
| TDR | 117,838,246 | 117,838,265 | TGAGGCAGAAGGGCTACAAG |
PCR Primers
| Primer | Sequence |
| SU | GCCCGAGAATCACCTAGAAG |
| SD | TTGGTTGGTCTGTCTGTCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18176A-B2 | VGB6 | C57BL/6NTac | |
| 18176A-C11 | VGB6 | C57BL/6NTac | |
| 18176A-D1 | VGB6 | C57BL/6NTac | |
| 18176A-D11 | VGB6 | C57BL/6NTac | |
| 18176A-F7 | VGB6 | C57BL/6NTac | |
| 18176A-G8 | VGB6 | C57BL/6NTac | |
