| Gene | Tdp1 |
| Chromosome | 12 |
| Deletion Start | 99,915,552 |
| Deletion End | 99,891,187 |
| Deletion Size | 24,366 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,891,195 | 99,891,215 | AAGCAGCTACGGGAAGTGGAC |
| TUP | 99,891,219 | 99,891,245 | ATCCAGTAGTGACGAAAGCGAGGATGA |
| TUR | 99,891,252 | 99,891,272 | GCTGACGGCTTATCAGGTTTG |
| TDF | 99,915,000 | 99,915,020 | GGGCTGCATTCCACTCACTTG |
| TDP | 99,915,022 | 99,915,045 | TCTCACTGGAGAAGCTGCTGTGGA |
| TDR | 99,915,078 | 99,915,098 | TCTCGGAAGCAGGAAGCTAAC |
PCR Primers
| Primer | Sequence |
| SU | TGGCCTTAACCTTACCTCCC |
| SD | TTACCCTACATGTCAGTTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18180A-H1 | VGB6 | C57BL/6NTac | |
| 18180B-H7 | VGB6 | C57BL/6NTac | |
