| Gene | Wipf2 |
| Chromosome | 11 |
| Deletion Start | 98,899,749 |
| Deletion End | 98,885,045 |
| Deletion Size | 14,705 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,885,116 | 98,885,137 | AGCCAGTGGGCTATCTTCAAAC |
| TUP | 98,885,142 | 98,885,169 | AAGTAAACTGGTGGGCTTCTCGTACTGG |
| TUR | 98,885,223 | 98,885,245 | GCTCACAGTGAAAGAGCAGTTAG |
| TDF | 98,899,663 | 98,899,682 | CTCTCCCACCCATTCTCAGG |
| TDP | 98,899,683 | 98,899,707 | TGAAGCTTGGCGTAGTCCTGCTCCT |
| TDR | 98,899,710 | 98,899,729 | GAGGAGGTCCATCCTTTTCC |
PCR Primers
| Primer | Sequence |
| SU | GCTGGGAATAGAGATCCGTG |
| SD | TGCATCATGGAACACCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 130 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18181A-D2 | VGB6 | C57BL/6NTac | |
| 18181B-D7 | VGB6 | C57BL/6NTac | |
