| Gene | Avp |
| Chromosome | 2 |
| Deletion Start | 130,582,538 |
| Deletion End | 130,580,626 |
| Deletion Size | 1,913 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,582,519 | 130,582,536 | CGCCAGGATGCTCAACAC |
| TUP | 130,582,494 | 130,582,517 | ACGCTCTCCGCTTGTTTCCTGAGC |
| TUR | 130,582,449 | 130,582,470 | TCTTGGGCAGTTCTGGAAGTAG |
| TDF | 130,581,398 | 130,581,416 | TGATGACCCAGCCGGTTAG |
| TDP | 130,581,372 | 130,581,395 | TTTCACTCTTGCCCTTACCGCTGC |
| TDR | 130,581,344 | 130,581,362 | CTGGTGCTCTGGCGATGTC |
PCR Primers
| Primer | Sequence |
| SU | ACCTCACTGATCGCACAGC |
| SD | TATTCCGGTCTCAGCCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18182A-A4 | VGB6 | C57BL/6NTac | |
| 18182A-A6 | VGB6 | C57BL/6NTac | |
| 18182A-B7 | VGB6 | C57BL/6NTac | |
| 18182A-F6 | VGB6 | C57BL/6NTac | |
| 18182A-F10 | VGB6 | C57BL/6NTac | |
