| Gene | Dctn2 |
| Chromosome | 10 |
| Deletion Start | 127,281,544 |
| Deletion End | 127,266,502 |
| Deletion Size | 15,043 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,267,303 | 127,267,324 | GGGCGGAATCCTTGAATGACAG |
| TUP | 127,267,333 | 127,267,360 | TGCAATGCCTTGTTCAAAGGAAATGGGA |
| TUR | 127,267,383 | 127,267,402 | AGCGGTGACCACCCAGACTC |
| TDF | 127,280,696 | 127,280,719 | GAGTCATGTAGACCCTGACCTAAG |
| TDP | 127,280,720 | 127,280,743 | TCCACAGCACTCAGAGGGTTGCTT |
| TDR | 127,280,762 | 127,280,783 | TGGGCGGATAGGTAAAGAAGTG |
PCR Primers
| Primer | Sequence |
| SU | CTACATATCCCACAATGCTAG |
| SD | TCTCCGAAATCCAGGAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18184A-D9 | VGB6 | C57BL/6NTac | |
| 18184A-G9 | VGB6 | C57BL/6NTac | |
| 18184A-F8 | VGB6 | C57BL/6NTac | |
| 18184A-F1 | VGB6 | C57BL/6NTac | |
