| Gene | Cdc42se1 |
| Chromosome | 3 |
| Deletion Start | 95,233,668 |
| Deletion End | 95,232,042 |
| Deletion Size | 1,627 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,232,437 | 95,232,461 | TGCCAAATCAGTTCATAGCTCTTAG |
| TUP | 95,232,468 | 95,232,495 | TTCAAGACTCCTTCTCCTTCCTCTGCTG |
| TUR | 95,232,503 | 95,232,521 | GTGCGTATGCAGCCATCTC |
| TDF | 95,232,860 | 95,232,883 | GTGGTCTCCCTTCCTATTAAGAAC |
| TDP | 95,232,887 | 95,232,909 | TCAGAGCCAGGCCGTAGAGTGCT |
| TDR | 95,232,933 | 95,232,951 | GCAGTGCTGGAGAGCAAAC |
PCR Primers
| Primer | Sequence |
| SU | AGAGAGGGTTGTTTCCCACC |
| SD | GGGAGTCACAAGGAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18187A-B8 | VGB6 | C57BL/6NTac | |
| 18187A-B9 | VGB6 | C57BL/6NTac | |
| 18187A-C9 | VGB6 | C57BL/6NTac | |
| 18187A-H1 | VGB6 | C57BL/6NTac | |
| 18187A-E9 | VGB6 | C57BL/6NTac | |
| 18187A-G11 | VGB6 | C57BL/6NTac | |
