| Gene | Ngb |
| Chromosome | 12 |
| Deletion Start | 87,102,257 |
| Deletion End | 87,098,380 |
| Deletion Size | 3,878 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,101,743 | 87,101,765 | TCGAATGGAAGGAGACCATGTAG |
| TUP | 87,101,717 | 87,101,741 | CCTGGCCCAGGAGAACTCAAGTTCA |
| TUR | 87,101,684 | 87,101,705 | CCTAAGCCTGTGCTTTCTCATC |
| TDF | 87,098,917 | 87,098,939 | GGTCATCCAGGCTACGAGTATTG |
| TDP | 87,098,881 | 87,098,908 | TCTTAACTCACACCTAGCTGGCTCCACA |
| TDR | 87,098,831 | 87,098,850 | CCGATTCCCTGACTGCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GGCTCGGCAATTTCTGTTAG |
| SD | GCTTGGCAGGGAAGTATCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18189A-A3 | VGB6 | C57BL/6NTac | |
| 18189A-B4 | VGB6 | C57BL/6NTac | |
| 18189A-B9 | VGB6 | C57BL/6NTac | |
| 18189A-H12 | VGB6 | C57BL/6NTac | |
| 18189A-D4 | VGB6 | C57BL/6NTac | |
| 18189A-B10 | VGB6 | C57BL/6NTac | |
