| Gene | Pomt2 |
| Chromosome | 12 |
| Deletion Start | 87,147,625 |
| Deletion End | 87,109,124 |
| Deletion Size | 38,502 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,147,211 | 87,147,230 | GGTGACGCTGCTGTCCTTTG |
| TUP | 87,147,187 | 87,147,208 | ACCCGCTTTCACCGGCTGGACC |
| TUR | 87,147,151 | 87,147,173 | AAGTCCTTTCCGCCACTTACCAG |
| TDF | 87,109,278 | 87,109,299 | TCACAGCTTCTACCTCTTCCAC |
| TDP | 87,109,252 | 87,109,277 | CCTCTGGCTTATGGGATGGTTGGTCC |
| TDR | 87,109,200 | 87,109,219 | TGATTCCAGCCACCTCAGTC |
PCR Primers
| Primer | Sequence |
| SU | CTCTTCTCCGCGTTTCTCAG |
| SD | AACCTTGGACCATTTCTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18190A-C5 | VGB6 | C57BL/6NTac | |
| 18190A-D1 | VGB6 | C57BL/6NTac | |
| 18190A-H6 | VGB6 | C57BL/6NTac | |
