| Gene | Acsbg2 |
| Chromosome | 17 |
| Deletion Start | 56,872,848 |
| Deletion End | 56,845,364 |
| Deletion Size | 27,485 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,872,697 | 56,872,719 | TGTCCCGTTCTTCCTGGTTATTG |
| TUP | 56,872,666 | 56,872,694 | TATGAAGTAGCACGTGGTCAGCAGTTTGG |
| TUR | 56,872,611 | 56,872,630 | CCTGTGCTGTGCCTCTTGAC |
| TDF | 56,845,961 | 56,845,980 | CAGGGCAGACACCTTTACAC |
| TDP | 56,845,935 | 56,845,960 | TCTGGAGGTTACATCCTCACGGTGGG |
| TDR | 56,845,883 | 56,845,904 | GGTCCTCGATAAGGGCAAACAG |
PCR Primers
| Primer | Sequence |
| SU | ATGGTGAGCTGACTGGCTG |
| SD | ACAGAATTTCCAGAGCCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18191A-B3 | VGB6 | C57BL/6NTac | |
| 18191A-D4 | VGB6 | C57BL/6NTac | |
| 18191A-D11 | VGB6 | C57BL/6NTac | |
| 18191A-D7 | VGB6 | C57BL/6NTac | |
