| Gene | Thap3 |
| Chromosome | 4 |
| Deletion Start | 151,988,953 |
| Deletion End | 151,983,003 |
| Deletion Size | 5,951 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 151,988,460 | 151,988,477 | CTCGTGCTGAGCCCTTTC |
| TUP | 151,988,435 | 151,988,458 | AGGGAAGCACCCTTCAACTGCTCC |
| TUR | 151,988,394 | 151,988,413 | CCAGGAACTGAGCCCTGAAG |
| TDF | 151,983,395 | 151,983,414 | GCTCATGCCGTCCTGCTTAG |
| TDP | 151,983,369 | 151,983,393 | ACACAAGGCTCTTCCCAGCACTCAC |
| TDR | 151,983,320 | 151,983,339 | CGTGGCCTCCATTGCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | CGGACAAACCCAGCCCTG |
| SD | AGCATAACCGAGTGACAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18207A-A1 | VGB6 | C57BL/6NTac | |
| 18207A-D3 | VGB6 | C57BL/6NTac | |
| 18207A-A9 | VGB6 | C57BL/6NTac | |
| 18207A-B11 | VGB6 | C57BL/6NTac | |
