18211   Ccdc127     Status: ES cell colonies screened / QC positives

GeneCcdc127
Chromosome13
Deletion Start74,357,124
Deletion End74,352,869
Deletion Size4,256
Genome Build38
MGI IDMGI:1914683
NCBI Gene ID67433
BAC ID321K12
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 13

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,353,60174,353,625TCGCATCCTATGTGATTTCAAGAAG
TUP74,353,62674,353,653TCACAGTGTAGTGTGGGAGGCATGTCTC
TUR74,353,72274,353,742TGACAACCAGGACCACAAGAC
TDF74,356,91074,356,929GCGCACATCTGTTGATCCAG
TDP74,356,93074,356,952TGGCTGCTGACCTAGAAATGGCA
TDR74,356,99074,357,010TGGTGTTCCAGACATCTCCAC

PCR Primers

PrimerSequence
SUGGTTTGTGATGGTATGTGTGC
SDCATGAAATCAGGAAAGGGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR142 bp++-
TDFTDR101 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
18211A-A10VGB6C57BL/6NTac
18211A-E5VGB6C57BL/6NTac
18211A-G9VGB6C57BL/6NTac
18211A-F9VGB6C57BL/6NTac
18211A-F4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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