| Gene | Mknk1 |
| Chromosome | 4 |
| Deletion Start | 115,878,194 |
| Deletion End | 115,857,037 |
| Deletion Size | 21,158 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,857,391 | 115,857,408 | CACTTGTGCCTGCTCCTC |
| TUP | 115,857,410 | 115,857,435 | TCAGATGTGAGCCTTCTTCCTGTGGT |
| TUR | 115,857,440 | 115,857,460 | TCTTCTGCTTTCCAGCCTTCC |
| TDF | 115,877,713 | 115,877,733 | CAACGGTAGAGCCTGGAAGTG |
| TDP | 115,877,736 | 115,877,756 | TGCTCTGACTGCCCTGCCCAT |
| TDR | 115,877,796 | 115,877,815 | CGCAGCTTTGCCTGGTTTAG |
PCR Primers
| Primer | Sequence |
| SU | TGCAGTGATGGAGATAACGC |
| SD | AGCTCCTGGAAGCTGGACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18214B-F8 | VGB6 | C57BL/6NTac | |
