| Gene | 2310030N02Rik |
| Chromosome | 4 |
| Deletion Start | 11,076,173 |
| Deletion End | 11,051,094 |
| Deletion Size | 25,080 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,075,843 | 11,075,862 | CCGTGCTTGGAGAAGGAGAC |
| TUP | 11,075,817 | 11,075,839 | AGGCCTGTTCACGTGGCTCAGCA |
| TUR | 11,075,795 | 11,075,816 | GTTAGGGTTCAGGCAGAGGATG |
| TDF | 11,051,934 | 11,051,952 | TTTGTGGTGGCACACACTG |
| TDP | 11,051,906 | 11,051,929 | TCCACTGCTCCAGAAGCTAAGGGA |
| TDR | 11,051,873 | 11,051,895 | CATCCCAGACTGGCCTCAAATAC |
PCR Primers
| Primer | Sequence |
| SU | GCTATGCTGGGAGGTCTCTG |
| SD | GAAGTTCTGCAGCTGATCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18217A-A6 | VGB6 | C57BL/6NTac | |
| 18217A-B5 | VGB6 | C57BL/6NTac | |
| 18217A-G10 | VGB6 | C57BL/6NTac | |
| 18217A-F1 | VGB6 | C57BL/6NTac | |
| 18217A-F8 | VGB6 | C57BL/6NTac | |
