| Gene | BC048679 |
| Chromosome | 7 |
| Deletion Start | 81,496,864 |
| Deletion End | 81,495,086 |
| Deletion Size | 1,779 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,496,181 | 81,496,199 | CCTTGGCCCAACCTCACTG |
| TUP | 81,496,144 | 81,496,171 | TGTTTCTTCAGTCCCTCATCTCAACCCA |
| TUR | 81,496,109 | 81,496,127 | CAAGCAACACCGCCTTCTG |
| TDF | 81,495,204 | 81,495,222 | CCGCGTGCGAGAAGCTATG |
| TDP | 81,495,173 | 81,495,200 | AACGATCCTCCTCCCTACCATCCCACTA |
| TDR | 81,495,136 | 81,495,158 | CCCAACAGGTTCAGTGCAAAGTG |
PCR Primers
| Primer | Sequence |
| SU | ACAGGGATGCCCAGTTCTC |
| SD | GGAAGTATTCCCAAGGACCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18218A-C3 | VGB6 | C57BL/6NTac | |
| 18218A-E9 | VGB6 | C57BL/6NTac | |
