| Gene | Ddx55 |
| Chromosome | 5 |
| Deletion Start | 124,568,735 |
| Deletion End | 124,552,935 |
| Deletion Size | 15,801 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,553,424 | 124,553,445 | TGGTGTGTATCCAGGTCTCATC |
| TUP | 124,553,447 | 124,553,476 | AGGCTCCAGAAACTTGGCAGACTTGATACC |
| TUR | 124,553,502 | 124,553,524 | GCCCGTTCCTATGGTTCTTTATC |
| TDF | 124,567,794 | 124,567,811 | CCCGTGCTCTTTGCTGAC |
| TDP | 124,567,820 | 124,567,849 | TTGCTTTGCTTTCAGATCTTGACTTTGCCG |
| TDR | 124,567,873 | 124,567,891 | GCATCCTGGGCATCCTGAG |
PCR Primers
| Primer | Sequence |
| SU | TCCTATGTGCACCGGCGG |
| SD | CAAGTATACTTCCTTATCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18220A-E2 | VGB6 | C57BL/6NTac | |
| 18220A-G9 | VGB6 | C57BL/6NTac | |
