| Gene | Tbc1d22b |
| Chromosome | 17 |
| Deletion Start | 29,578,939 |
| Deletion End | 29,549,942 |
| Deletion Size | 28,998 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,550,369 | 29,550,388 | GGTTCCCAGCCCATCATCAC |
| TUP | 29,550,391 | 29,550,414 | TCCTGTCTTCCCTTGGACTCCCAA |
| TUR | 29,550,424 | 29,550,442 | TGGCCCAGGCTCTCCTATC |
| TDF | 29,578,380 | 29,578,399 | GGCTACTGAGCCACCTCTTC |
| TDP | 29,578,404 | 29,578,430 | CCCAGAACGGAATCTTGACCTCTTCCC |
| TDR | 29,578,453 | 29,578,473 | AACCACCACTTGCCACCATTC |
PCR Primers
| Primer | Sequence |
| SU | CAGCGGCCCGCAGGATCC |
| SD | GAGACATGTTTAGAGAGGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18226B-E3 | VGB6 | C57BL/6NTac | |
