| Gene | Exoc7 |
| Chromosome | 11 |
| Deletion Start | 116,306,670 |
| Deletion End | 116,289,152 |
| Deletion Size | 17,519 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,306,496 | 116,306,513 | TGCTGGTGGTCGCCTTTC |
| TUP | 116,306,469 | 116,306,494 | CCATCGGCTTGTGGTGATGTCCTCCT |
| TUR | 116,306,416 | 116,306,436 | TCCAGGCTGTCTCGGATAAAC |
| TDF | 116,289,694 | 116,289,716 | CCATACCCAGCCTTAACTTTGTC |
| TDP | 116,289,668 | 116,289,693 | TCTTGGTCCCTCCTTCCTACCTGTGT |
| TDR | 116,289,629 | 116,289,649 | GCACAGCTCTTCGAGTCCATC |
PCR Primers
| Primer | Sequence |
| SU | TCAGGTATAATGTTGGTTTGCTTG |
| SD | CCTAAGAGAGTGTGGCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18229A-B4 | VGB6 | C57BL/6NTac | |
| 18229A-C1 | VGB6 | C57BL/6NTac | |
