| Gene | Nr1i2 |
| Chromosome | 16 |
| Deletion Start | 38,266,098 |
| Deletion End | 38,249,195 |
| Deletion Size | 16,904 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GAGAGAGAGAGAGAGAGATCCTTC |
| TUP | 38,265,364 | 38,265,391 | TCACAGCATGTTAGGTCTGGACCAAAGG |
| TUR | 38,265,311 | 38,265,331 | TGGCTTTGCTGATTGTCACTG |
| TDF | 38,249,380 | 38,249,400 | GCTGCCTACAGGTTCCTGTTC |
| TDP | 38,249,354 | 38,249,378 | TGAAGATCATGGCCGTCCTCACTGA |
| TDR | 38,249,313 | 38,249,331 | GCAGCAACTGCTGGGTTTG |
PCR Primers
| Primer | Sequence |
| SU | TAGTCCAATCCAGGTAGCGG |
| SD | GAACCTGAAGATCAGCTCGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18230A-B4 | VGB6 | C57BL/6NTac | |
| 18230A-F6 | VGB6 | C57BL/6NTac | |
