| Gene | Prune |
| Chromosome | 3 |
| Deletion Start | 95,281,620 |
| Deletion End | 95,254,975 |
| Deletion Size | 26,646 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,280,731 | 95,280,750 | GCTCGGTATGGTAGCCACAG |
| TUP | 95,280,702 | 95,280,729 | CCTCTAGTTCCAACTCAGAGGAGGCTGA |
| TUR | 95,280,682 | 95,280,701 | ACCTGGCCTGAACTGAAGTC |
| TDF | 95,255,433 | 95,255,456 | CCATCATCTCCTGTTCTTGTCTTC |
| TDP | 95,255,400 | 95,255,427 | ATCTGTGGCATCCTGGAACGATCCACCT |
| TDR | 95,255,363 | 95,255,383 | GAGGTGCTTGGGATAGGTGTC |
PCR Primers
| Primer | Sequence |
| SU | GTTCAGATGCCATAAAGGCG |
| SD | TCCTTTAGTCCTGCTCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18232A-H6 | VGB6 | C57BL/6NTac | |
| 18232A-B10 | VGB6 | C57BL/6NTac | |
