| Gene | Dr1 |
| Chromosome | 5 |
| Deletion Start | 108,278,666 |
| Deletion End | 108,269,593 |
| Deletion Size | 9,074 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,270,004 | 108,270,025 | TCCGGCTAGTGCAAATAAGGAG |
| TUP | 108,270,028 | 108,270,055 | CAGACATTAGGGTCAACAGACATCCGCA |
| TUR | 108,270,096 | 108,270,116 | GCTGTGGGCAGTCAATTCTTG |
| TDF | 108,278,269 | 108,278,291 | GCTAGAAATCAACCCTGATCCTC |
| TDP | 108,278,294 | 108,278,323 | TGTAAGAAACCCAGTACTCCTAACTGCTGA |
| TDR | 108,278,329 | 108,278,353 | AAAGGTTAATAGAGCGGCTGGAGAG |
PCR Primers
| Primer | Sequence |
| SU | GCGGGTATTCCTCTGTCTTG |
| SD | CTGCATCACATCATGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18236A-C2 | VGB6 | C57BL/6NTac | |
| 18236A-H11 | VGB6 | C57BL/6NTac | |
| 18236A-E9 | VGB6 | C57BL/6NTac | |
