| Gene | Hint1 |
| Chromosome | 11 |
| Deletion Start | 54,870,398 |
| Deletion End | 54,866,493 |
| Deletion Size | 3,906 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,866,983 | 54,867,004 | CGAGATCCTTATGCTCCCTGTC |
| TUP | 54,867,006 | 54,867,027 | CCTGCTCCGGCTGCAGACGTTG |
| TUR | 54,867,036 | 54,867,057 | GCTTGAAGGGCTAATTCCAGTC |
| TDF | 54,869,673 | 54,869,697 | AGGCTCCATCCTAAGACTAAGTTAC |
| TDP | 54,869,699 | 54,869,724 | TGCAAAGGATTGTGAGGCCATGTACA |
| TDR | 54,869,752 | 54,869,771 | GAATGGGAGGCACCAAACAG |
PCR Primers
| Primer | Sequence |
| SU | CACCCAGTAAATGTGATCAGAAC |
| SD | TATTCAATGCCAGGGTCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18237A-A6 | VGB6 | C57BL/6NTac | |
| 18237A-H1 | VGB6 | C57BL/6NTac | |
