| Gene | Lnx2 |
| Chromosome | 5 |
| Deletion Start | 147,042,437 |
| Deletion End | 147,018,898 |
| Deletion Size | 23,540 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 147,042,110 | 147,042,128 | CACAAGCTGCTGGACAAAC |
| TUP | 147,042,086 | 147,042,109 | TGCTGGTCCTGTGTCCGTTCTCTC |
| TUR | 147,042,064 | 147,042,083 | TGCATCACATCTTGGCACAC |
| TDF | 147,019,674 | 147,019,692 | GTGCCTCAGAATGGCAGTG |
| TDP | 147,019,650 | 147,019,672 | CCTCTGAGCGTTTCCCATGCAGC |
| TDR | 147,019,607 | 147,019,627 | CGAGCCTCGGGAAATATCAAC |
PCR Primers
| Primer | Sequence |
| SU | GACAGTGAGGTGCTAGCGTG |
| SD | TTGAAATGTTGGCACCAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18239A-A4 | VGB6 | C57BL/6NTac | |
| 18239A-B8 | VGB6 | C57BL/6NTac | |
| 18239A-H11 | VGB6 | C57BL/6NTac | |
