| Gene | Hspa12a |
| Chromosome | 19 |
| Deletion Start | 58,860,886 |
| Deletion End | 58,825,256 |
| Deletion Size | 35,631 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,859,978 | 58,859,996 | GCTTTCCCAGCCAGAGTTG |
| TUP | 58,859,953 | 58,859,974 | ATCAAGGCTTCGCCGCCCGCAC |
| TUR | 58,859,907 | 58,859,927 | TGAGATGCACAGGAAGAAGAC |
| TDF | 58,826,170 | 58,826,191 | GCATGACCTCTGCACCTACCTG |
| TDP | 58,826,146 | 58,826,167 | TTTGGGCTCAACAGCAGCGTCC |
| TDR | 58,826,084 | 58,826,101 | TGGAACCGGGCTGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGTGGACTGGAGTGTGCTTC |
| SD | CAGCTGGGATGCTCTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18244A-G1 | VGB6 | C57BL/6NTac | |
| 18244A-E1 | VGB6 | C57BL/6NTac | |
