| Gene | Tk1 |
| Chromosome | 11 |
| Deletion Start | 117,825,776 |
| Deletion End | 117,815,865 |
| Deletion Size | 9,912 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,825,083 | 117,825,105 | AGCGTCTTTATTTGTGGCCCAAC |
| TUP | 117,825,059 | 117,825,082 | TCAGAGATCCGCTTGCCTCTGCTG |
| TUR | 117,825,003 | 117,825,023 | TGAGGTGACACCAGACCATTC |
| TDF | 117,816,563 | 117,816,581 | CTGTCTTCCGTGCAGGCTT |
| TDP | 117,816,539 | 117,816,561 | CGGCAGCATCTTGAACCTGGTGC |
| TDR | 117,816,497 | 117,816,515 | CCATGCACACAGCGGTGAG |
PCR Primers
| Primer | Sequence |
| SU | GAAGAAATCTGGCGCTCAAC |
| SD | CCAGGCTAAGGAGAAACACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18248A-A8 | VGB6 | C57BL/6NTac | |
| 18248A-C8 | VGB6 | C57BL/6NTac | |
| 18248A-C9 | VGB6 | C57BL/6NTac | |
| 18248A-D1 | VGB6 | C57BL/6NTac | |
| 18248A-D3 | VGB6 | C57BL/6NTac | |
