| Gene | Arf3 |
| Chromosome | 15 |
| Deletion Start | 98,742,737 |
| Deletion End | 98,740,306 |
| Deletion Size | 2,432 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,742,404 | 98,742,422 | CACTCAGCAGTGCCCTCAG |
| TUP | 98,742,369 | 98,742,398 | AGGCTTGGTTTGTTCTCTGTGTTGTTTGGA |
| TUR | 98,742,333 | 98,742,354 | GCCTCTGGCAGGAACATCAAAG |
| TDF | 98,740,838 | 98,740,860 | GCTTCTTCATGCTCTTCTCCTTG |
| TDP | 98,740,813 | 98,740,834 | CCCAGCCCAGCTGTGTCAGCAG |
| TDR | 98,740,769 | 98,740,790 | TCCTGAACAGACATGCTACCAC |
PCR Primers
| Primer | Sequence |
| SU | TTCAGTCTGGGTGTGAGTGG |
| SD | CAGTGCCAAACAGCAAACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18250A-E1 | VGB6 | C57BL/6NTac | |
