| Gene | Pkd2l2 |
| Chromosome | 18 |
| Deletion Start | 34,438,891 |
| Deletion End | 34,409,470 |
| Deletion Size | 29,422 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,409,502 | 34,409,524 | GGGTGGGTACGCTTAAAGGATAG |
| TUP | 34,409,526 | 34,409,547 | TAACCCAGGGCCCATTGGCAAC |
| TUR | 34,409,585 | 34,409,604 | TGGCAACCATCACAGGAGTG |
| TDF | 34,438,394 | 34,438,417 | GCTGGAGAGATGATTCGGTGATTG |
| TDP | 34,438,419 | 34,438,441 | CAGCACTGGCTGCCCTTCCTGAG |
| TDR | 34,438,478 | 34,438,501 | TCCTGGGACTGGAATTAGAATGTG |
PCR Primers
| Primer | Sequence |
| SU | TGGCCACTCATGTTAATACTGC |
| SD | AGTGTGCCCGTGTACAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18256A-C12 | VGB6 | C57BL/6NTac | |
| 18256A-D8 | VGB6 | C57BL/6NTac | |
| 18256A-F4 | VGB6 | C57BL/6NTac | |
| 18256A-F9 | VGB6 | C57BL/6NTac | |
