| Gene | Taf6 |
| Chromosome | 5 |
| Deletion Start | 138,184,484 |
| Deletion End | 138,178,767 |
| Deletion Size | 5,718 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,184,414 | 138,184,434 | CATGAAGGTGGTGGCAGAATC |
| TUP | 138,184,387 | 138,184,412 | ATGGGCATTGCTCAGATTCAGGAGGA |
| TUR | 138,184,342 | 138,184,361 | TCCTTGATGCGGTAGCTGAC |
| TDF | 138,179,682 | 138,179,700 | TCCCACGCCCAATGAAGAG |
| TDP | 138,179,651 | 138,179,674 | CCTGGAGTGGCTCAGGTCTGCCTT |
| TDR | 138,179,604 | 138,179,625 | GCCGAGGCTATGAAGAGAACAG |
PCR Primers
| Primer | Sequence |
| SU | AGAAAGGACAGGCCAAGCTG |
| SD | AATCCTCACAAGTGGGTTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18258A-G7 | VGB6 | C57BL/6NTac | |
