| Gene | Tmem214 |
| Chromosome | 5 |
| Deletion Start | 30,876,857 |
| Deletion End | 30,869,671 |
| Deletion Size | 7,187 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,869,908 | 30,869,927 | CCACGGTGACTCAGGGACAG |
| TUP | 30,869,929 | 30,869,953 | ACGAGGAGAGGTGGTCATCTCCGCT |
| TUR | 30,869,955 | 30,869,975 | GACCTCCGCTTCCGACCTATG |
| TDF | 30,876,683 | 30,876,707 | TGCATTAAGACACAGTTGAGCAGAG |
| TDP | 30,876,708 | 30,876,730 | CTGCCCAATGGACCTGGCTCTGC |
| TDR | 30,876,750 | 30,876,770 | TGAGTGCCCAGTCCAAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CAATTAAGTTTGTTGGCCGC |
| SD | AGTCATGACCAGGGACAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18259A-F11 | VGB6 | C57BL/6NTac | |
| 18259A-B10 | VGB6 | C57BL/6NTac | |
| 18259A-F4 | VGB6 | C57BL/6NTac | |
| 18259A-D5 | VGB6 | C57BL/6NTac | |
| 18259A-C12 | VGB6 | C57BL/6NTac | |
| 18259A-F8 | VGB6 | C57BL/6NTac | |
