| Gene | Ap4m1 |
| Chromosome | 5 |
| Deletion Start | 138,178,550 |
| Deletion End | 138,172,194 |
| Deletion Size | 6,357 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,172,855 | 138,172,877 | AAACGTCTCTCCTTTCAGCCTTC |
| TUP | 138,172,878 | 138,172,897 | TGGAGCTGCTTTCCCGGTGA |
| TUR | 138,172,898 | 138,172,916 | GTGCCTGTCCCAACTCTAC |
| TDF | 138,178,217 | 138,178,236 | CCAGTCCTGCTGCCGAGTAG |
| TDP | 138,178,238 | 138,178,264 | TGTCTAGCCTCACATCTTCCCTGACTC |
| TDR | 138,178,270 | 138,178,291 | GCCAGGGACATCCATCTGTAAG |
PCR Primers
| Primer | Sequence |
| SU | GTGTGGCAGGACTTGAAGG |
| SD | TCTCATCCTTCTCCAGTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18260A-H4 | VGB6 | C57BL/6NTac | |
| 18260A-F8 | VGB6 | C57BL/6NTac | |
