| Gene | Npat |
| Chromosome | 9 |
| Deletion Start | 53,572,692 |
| Deletion End | 53,537,155 |
| Deletion Size | 35,538 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,537,298 | 53,537,316 | TGCTCCCGTCCAAGTTCAG |
| TUP | 53,537,317 | 53,537,339 | ACTAGCGGAGAGGCTCGGTCCCT |
| TUR | 53,537,376 | 53,537,395 | GCAGGAGGAGAAGGGACATC |
| TDF | 53,571,887 | 53,571,906 | GTCCTGCAAGCCTGAGTTTG |
| TDP | 53,571,912 | 53,571,940 | TCTTATCAAGTGTATGCCATTCCTGTGCC |
| TDR | 53,571,961 | 53,571,981 | AGGCACAGAGGAAGAGACCAG |
PCR Primers
| Primer | Sequence |
| SU | AGGTCCTCTCTGGCCACAAC |
| SD | AGTCAATCAAGACAGTGTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18262A-A9 | VGB6 | C57BL/6NTac | |
| 18262A-C12 | VGB6 | C57BL/6NTac | |
| 18262A-E11 | VGB6 | C57BL/6NTac | |
| 18262A-H2 | VGB6 | C57BL/6NTac | |
