| Gene | Terf2 |
| Chromosome | 8 |
| Deletion Start | 107,096,506 |
| Deletion End | 107,070,406 |
| Deletion Size | 26,101 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,096,028 | 107,096,049 | GAGCCAACCATGTCTTCTTTCC |
| TUP | 107,096,005 | 107,096,027 | CAGCGTTGCTTGTCAGGCCCTTG |
| TUR | 107,095,965 | 107,095,982 | GCATAACCCGCAGCAACC |
| TDF | 107,070,674 | 107,070,695 | CACACTCGGCTTGTAGCTCATC |
| TDP | 107,070,642 | 107,070,669 | ACCCTGCTGTTGGGTTCAGGATTGTTGA |
| TDR | 107,070,599 | 107,070,618 | TGTTGCCGCTCACCTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | TTTCTACTGTACTGCTTTTCAC |
| SD | TTGAAGCAAAGCACTAAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18263B-D1 | VGB6 | C57BL/6NTac | |
| 18263B-H11 | VGB6 | C57BL/6NTac | |
| 18263A-B7 | VGB6 | C57BL/6NTac | |
