| Gene | Cdc7 |
| Chromosome | 5 |
| Deletion Start | 106,983,376 |
| Deletion End | 106,965,021 |
| Deletion Size | 18,356 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,965,105 | 106,965,129 | AAACTTTCAGGTATGTGCTCAATGC |
| TUP | 106,965,130 | 106,965,154 | CACGGTCACACGGTTCTGAAGTCGC |
| TUR | 106,965,163 | 106,965,187 | CCTCGAATTAGAAGATGCAGACTGG |
| TDF | 106,983,061 | 106,983,080 | CAGTGGTCCTCCAGGGAATG |
| TDP | 106,983,083 | 106,983,107 | TCCTATGACCCTGCTGCTTCCAAGA |
| TDR | 106,983,111 | 106,983,129 | CGGGATGCTTTGTGGTCAG |
PCR Primers
| Primer | Sequence |
| SU | TTATCCTTCTGTTGGCACCC |
| SD | GCACACTTTCCACACACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18266A-A9 | VGB6 | C57BL/6NTac | |
| 18266A-D5 | VGB6 | C57BL/6NTac | |
