| Gene | Dlgap3 |
| Chromosome | 4 |
| Deletion Start | 127,214,991 |
| Deletion End | 127,194,616 |
| Deletion Size | 20,376 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,194,748 | 127,194,766 | CGCTTCTGTGCTCCGAGAG |
| TUP | 127,194,767 | 127,194,789 | CTGGCCTGGGACACCTTTCTCCA |
| TUR | 127,194,818 | 127,194,838 | CCTCAGGGCCTACTGATGATG |
| TDF | 127,214,205 | 127,214,222 | TCGCAGCTACCGTAGACC |
| TDP | 127,214,224 | 127,214,247 | AGGTGCTCTCCAAAGCAAAGCTCC |
| TDR | 127,214,274 | 127,214,297 | GGGACCAGGAGGACCTTTATTGAG |
PCR Primers
| Primer | Sequence |
| SU | AAGCCCATTTGGCTGAAAG |
| SD | GCTAGGTACCAAGCTAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18268A-B10 | VGB6 | C57BL/6NTac | |
| 18268A-C1 | VGB6 | C57BL/6NTac | |
| 18268A-G2 | VGB6 | C57BL/6NTac | |
