| Gene | Prdx6 |
| Chromosome | 1 |
| Deletion Start | 161,251,113 |
| Deletion End | 161,241,640 |
| Deletion Size | 9,474 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 161,250,355 | 161,250,372 | GGGAGCACCCAGAAACAC |
| TUP | 161,250,329 | 161,250,354 | TCTGTAGGCGTGACCGTGATTCTAGA |
| TUR | 161,250,269 | 161,250,289 | GCCTTGGAATGGCCTAGAGTG |
| TDF | 161,242,415 | 161,242,439 | GCACTCTACCTTCTGACTCCATCTC |
| TDP | 161,242,391 | 161,242,413 | ACGGAGGCTGGCCTTGGACCCTT |
| TDR | 161,242,354 | 161,242,372 | GTCCGTCACCTCAGCACAC |
PCR Primers
| Primer | Sequence |
| SU | AACCCAGGTTGGCTGAGTAG |
| SD | CTAGCACAGAGCCCACACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18273A-B7 | VGB6 | C57BL/6NTac | |
