| Gene | 1700011F14Rik |
| Chromosome | 19 |
| Deletion Start | 58,537,359 |
| Deletion End | 58,512,361 |
| Deletion Size | 24,999 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,512,545 | 58,512,565 | CACATGCCAACAGCTCCTAAG |
| TUP | 58,512,567 | 58,512,595 | TGGAGGCTTCAGAAAGGTGAATGCAAGTA |
| TUR | 58,512,627 | 58,512,646 | TCCCACGACATCTGGTAATC |
| TDF | 58,536,859 | 58,536,879 | ACTGGGTGTAGGCTGCACAAG |
| TDP | 58,536,886 | 58,536,909 | AATGCGGATTGCGTTTAGGCTGCA |
| TDR | 58,536,941 | 58,536,959 | ATGGTGAGCGTGGGCACAG |
PCR Primers
| Primer | Sequence |
| SU | TGCCATGTCTGTTCTCCAAG |
| SD | TTGTGTAAGCATCCATGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18275A-A4 | VGB6 | C57BL/6NTac | |
| 18275A-G10 | VGB6 | C57BL/6NTac | |
| 18275A-B10 | VGB6 | C57BL/6NTac | |
| 18275A-D4 | VGB6 | C57BL/6NTac | |
