| Gene | Ankrd54 |
| Chromosome | 15 |
| Deletion Start | 79,062,794 |
| Deletion End | 79,053,939 |
| Deletion Size | 8,856 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,062,417 | 79,062,439 | GAGTGATCGCAGTGATCGTAGTG |
| TUP | 79,062,396 | 79,062,415 | TTCGAGCTGCGGAGGACCGT |
| TUR | 79,062,370 | 79,062,393 | TCAGGGATGAACAAGTGGATGAAG |
| TDF | 79,054,115 | 79,054,133 | TCCCTGCCCAGTGTGATAC |
| TDP | 79,054,090 | 79,054,113 | CGTGGATCAGCCTGGCTATCTCCC |
| TDR | 79,054,067 | 79,054,087 | GTCCGTCACTTCATCCACCTG |
PCR Primers
| Primer | Sequence |
| SU | GAAAGGAGTGTCCTGAACGC |
| SD | GCAATGTCCCTCTTCTGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18276A-B12 | VGB6 | C57BL/6NTac | |
| 18276A-C5 | VGB6 | C57BL/6NTac | |
