| Gene | Elk4 |
| Chromosome | 1 |
| Deletion Start | 132,023,662 |
| Deletion End | 132,014,395 |
| Deletion Size | 9,268 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 132,015,011 | 132,015,035 | CCCTCAGAACTAGAGCTACAAACAG |
| TUP | 132,015,037 | 132,015,062 | TGTTAACCACCTGACATGGGTGCTGG |
| TUR | 132,015,097 | 132,015,116 | GCTGGAGACAGGGCTAAGTG |
| TDF | 132,023,123 | 132,023,141 | CAGCCCTGGAGTACGGTTG |
| TDP | 132,023,144 | 132,023,168 | ACACCCAGGGACAGTTCATGGAGAA |
| TDR | 132,023,180 | 132,023,198 | TGGTAGCTGCTGGCAAAGG |
PCR Primers
| Primer | Sequence |
| SU | AAGCACTGTCAAAGCAAAACC |
| SD | AGGGTCCTAACATCCAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18277B-G3 | VGB6 | C57BL/6NTac | |
| 18277A-B7 | VGB6 | C57BL/6NTac | |
