| Gene | Ift81 |
| Chromosome | 5 |
| Deletion Start | 122,611,083 |
| Deletion End | 122,591,085 |
| Deletion Size | 19,999 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,610,624 | 122,610,644 | TGCTATCAAACCCGTGTGGAG |
| TUP | 122,610,593 | 122,610,622 | TCAGAGGACAACTTACAGGCATCAGTTTCC |
| TUR | 122,610,563 | 122,610,581 | GGCAAGGATCACAGCGAAC |
| TDF | 122,591,433 | 122,591,451 | CCCAGGGCCTTTGCCTTTC |
| TDP | 122,591,399 | 122,591,426 | TGAGTTTGACCATCCATGTTTGTCGTGG |
| TDR | 122,591,363 | 122,591,383 | CCTGCTCCTCTGGTCCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | CGGTTCTAATGTGGGTGGAC |
| SD | GAAACTCTGGAATACTGTTTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18278A-A11 | VGB6 | C57BL/6NTac | |
| 18278A-C2 | VGB6 | C57BL/6NTac | |
