| Gene | Pou2f3 |
| Chromosome | 9 |
| Deletion Start | 43,205,678 |
| Deletion End | 43,176,291 |
| Deletion Size | 29,388 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,205,321 | 43,205,340 | CCAGGAGGTTGCAGGAGTTG |
| TUP | 43,205,292 | 43,205,318 | TGGTTCAATCACTCAGCTAGGGTGTGG |
| TUR | 43,205,241 | 43,205,258 | TGCCCGGACACCAAACAC |
| TDF | 43,176,969 | 43,176,986 | GGTGTGGTGGCACAGAAC |
| TDP | 43,176,941 | 43,176,968 | TGTCCCAGCACTTAGGAGAAAGAAGGTA |
| TDR | 43,176,909 | 43,176,928 | TCCTGGGAATGGCTTTGAAC |
PCR Primers
| Primer | Sequence |
| SU | ACCTGGGGAGTGTGCTAAG |
| SD | GAGGCTCAGTCAATAGCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18280A-E12 | VGB6 | C57BL/6NTac | |
| 18280A-F11 | VGB6 | C57BL/6NTac | |
