| Gene | Pafah1b3 |
| Chromosome | 7 |
| Deletion Start | 25,297,449 |
| Deletion End | 25,295,026 |
| Deletion Size | 2,424 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,297,189 | 25,297,209 | GCGTGCCTTTGACTCTTTCTG |
| TUP | 25,297,161 | 25,297,186 | TTTCTCTGCAGCACCATCGGTTTGTA |
| TUR | 25,297,128 | 25,297,152 | GACTACTTCGGGTTCCTTGTCTTTG |
| TDF | 25,295,243 | 25,295,262 | GACCCTGGGTTTGTGCATTC |
| TDP | 25,295,213 | 25,295,242 | TGATGGCACCATAAGTCACCATGACATGTA |
| TDR | 25,295,191 | 25,295,211 | GCGGCTCAGGTGTAGGTAATC |
PCR Primers
| Primer | Sequence |
| SU | TAGATTGTCCCTGGTCCCTG |
| SD | CAGAGCACCAGGAAGTTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18284A-C5 | VGB6 | C57BL/6NTac | |
| 18284A-F12 | VGB6 | C57BL/6NTac | |
| 18284A-H4 | VGB6 | C57BL/6NTac | |
| 18284A-D3 | VGB6 | C57BL/6NTac | |
