| Gene | Pold1 |
| Chromosome | 7 |
| Deletion Start | 44,543,433 |
| Deletion End | 44,538,609 |
| Deletion Size | 4,825 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,542,701 | 44,542,719 | CAGGCGGGATGTGGTTCAC |
| TUP | 44,542,675 | 44,542,698 | CATTCCTCTCAGCGTGGGAGCCAG |
| TUR | 44,542,643 | 44,542,664 | ACCTGCTAAGGCCAGTTTACAG |
| TDF | 44,538,899 | 44,538,918 | AGGACGCCTTTCTGCCACTC |
| TDP | 44,538,872 | 44,538,898 | CGACTACTAGAGCGCCTTATGGTGCTG |
| TDR | 44,538,850 | 44,538,871 | GCGCCATCTCCACATTATTCAC |
PCR Primers
| Primer | Sequence |
| SU | ACCTGAGAGAAACAGCCAGG |
| SD | CGTCATAGTACCTGAGGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18286A-G7 | VGB6 | C57BL/6NTac | |
