| Gene | Ppfibp2 |
| Chromosome | 7 |
| Deletion Start | 107,653,227 |
| Deletion End | 107,637,250 |
| Deletion Size | 15,978 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,637,586 | 107,637,606 | GCGCATCGTGGCTTATTACTG |
| TUP | 107,637,607 | 107,637,634 | TGCAACTGAAGCTTGAAATTGAGCTGAC |
| TUR | 107,637,638 | 107,637,659 | GAGCAGACGCTGATTAGGGAAC |
| TDF | 107,653,096 | 107,653,116 | TGCCTGTGCTCCATCTCATTG |
| TDP | 107,653,120 | 107,653,147 | ACCTGAGACTAGCCTTGGAGATGTTGGC |
| TDR | 107,653,158 | 107,653,178 | GCTCAGGAGGGCTTCTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | AAATCAAATGAATGGAGCCG |
| SD | CCACAAGAAGGCAGAACGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18287C-B6 | VGB6 | C57BL/6NTac | |
