| Gene | Ccng1 |
| Chromosome | 11 |
| Deletion Start | 40,754,065 |
| Deletion End | 40,750,868 |
| Deletion Size | 3,198 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,754,035 | 40,754,057 | ACTGACAACTGACTCTCAGAAAC |
| TUP | 40,754,011 | 40,754,034 | TGCTACACCAGCTGAACACCCTGT |
| TUR | 40,753,976 | 40,753,995 | GCAGACCTTTGGCTGACATC |
| TDF | 40,750,949 | 40,750,973 | CGGTCAGAAGTTGAAATGGATTGTG |
| TDP | 40,750,928 | 40,750,947 | CTGGACGCACTGCACGGCAA |
| TDR | 40,750,880 | 40,750,900 | CTCAGGAATCGTTGGGAGGTG |
PCR Primers
| Primer | Sequence |
| SU | AGTTTGGGTGTATCATGTGGG |
| SD | GCACTGTACACAGTGAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18288B-F2 | VGB6 | C57BL/6NTac | |
| 18288B-F3 | VGB6 | C57BL/6NTac | |
| 18288A-F3 | VGB6 | C57BL/6NTac | |
